InvivoGen's siRNA Wizard™ is a program designed to help you select
siRNA/shRNA sequences targeting your gene(s) of interest.
This program selects siRNA/shRNA sequences that match criteria suggested by studies
of RNAi and which will have the best expression rate in psiRNA
vectors.
siRNA Wizard™ is composed of 3 parts:
- Find siRNA/shRNA sequence - Two types of searches
can be performed to find siRNA/shRNA sequences:
- "Standard search" utilizes a default set of criteria to analyze your gene of interest and provide the best sequences to silence gene expression.
- "Advanced search" lets you manually set the criteria for selecting the sequences against your target gene.
- Design hairpin insert - Using your selected siRNA/shRNA sequence, this tool will design two complementary oligonucleotides forming the insert needed to complete InvivoGen's psiRNA vector.
- Scramble siRNA/shRNA - Generate a negative control for siRNA/shRNA. This tool will returns a scrambled sequence with the same nucleotide composition as your siRNA/shRNA input sequence.
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